(K,L) Confocal images for Foxj1 (green in panels K,L and gray in panels K',L' ) immunofluorescence in Luc KD (K) or Jam3 KD (L) cells in ALI14. Mean relative to control cells and standard deviation as error bars were plotted for each lineage marker, n = 4. (H-J) mRNA expression levels of Krt5 (J), FoxJ1 (H), and Scgb1a1 (I) in Luc KD (C) and Jam3 KD (D) MTECs. (G) Jam3 immunofluorescence in BSCs during expansion in vitro, nucleus in blue (gray in panel G' ) and Jam3 in red (gray in panel G"" ). (D-F) Quantification of mRNA expression of Jam1 (E), Jam2 (F), and Jam3 (D) expression in ALI 0, ALI 4, ALI 7, and ALI14. (A-C) Quantification of mRNA expression of Krt5 (basal marker, in panel A ), FoxJ1 (multiciliated cells marker in panel B ), and Scgb1a1 (secretory cells marker in panel C ) expression in ALI 0, ALI 4, ALI 7, and ALI14. FIGURE 6 Junctional adhesion molecule 3 (Jam 3) expression varies along the differentiation process but does not affect cell differentiation.
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